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rat anti dectin 1 clec7a  (InvivoGen)


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    InvivoGen rat anti dectin 1 clec7a
    Rat Anti Dectin 1 Clec7a, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+dectin+1+clec7a/Anti-mDectin-1-IgG/pm42693199-712-83-86
    Average 96 stars, based on 37 article reviews
    rat anti dectin 1 clec7a - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    other:

    Article Title: Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice.
    Article Snippet: Brains were coronally sectioned at a thickness of 30 μM using a freezing sliding microtome (Leica, SM1020R).

    Cell-Signaling:

    Article Title: Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice
    Article Snippet: For each of the Iba1/CD68/Gfap and Iba1/Clec7a/Tmem119 co-stain panels, two hippocampus-containing sections were washed three times with TBS and incubated in primary antibodies diluted in blocking/permeabilization buffer comprised of 2% donkey serum (EMD Millipore, S30-100ML) and 0.25% Triton X-100/TBS overnight at 4C. .. The primary antibodies were diluted as follows: rat anti-CD68 (BioLegend, 137002, 1:250), goat anti-Iba1 (Novus Biologicals, NB100-1028, 1:1000), mouse anti-Gfap (eBioscience, 14-9892-82, 1:1000), rat anti-Dectin-1/Clec7a (Invivogen, mabg-mdect-2, 1:50), and rabbit anti-Tmem119 (Cell Signaling Technologies, 90840S, 1:500). ..

    Incubation:

    Article Title: Priming of CD8 + T cells by peripheral dendritic cells exacerbates tau-mediated neurodegeneration.
    Article Snippet: .. After washing, sections were blocked with a solution containing 10% normal donkey serum in TBSx for 1 h at RT, followed by an overnight incubation at 4 °C with primary antibodies (goat anti-Iba1 (Novus Biologicals, NB100-1028; 1:500), rat anti-MHC-II (BioLegend, 107603; 1:1,000 dilution), rabbit anti-P2RY12 (AnaSpec, AS-55043A; 1:1,000 dilution), Alexa Flour 488-conjugated mouse anti-GFAP (Novus Biologicals, NBP2-33184AF488,1:1,000 dilution), anti-Vimentin (Invitrogen, PA1-16759; 1:1,000 dilution), rabbit anti-MAP2 (Abcam, ab254264; 1:1,000 dilution), chicken anti-NeuN (Millipore, ABN91, 1:1,000 dilution), Armenian hamster anti-CD11c (Leinco, C6119, 1:400 dilution) and rat anti-Dectin-1 (Clec7a) (InvivoGen, mabg-mdect-2, 1:1,000 dilution)) in a TBSx solution containing 2% normal donkey serum. .. On the next day, after three washes in TBSx (10 min per cycle), brain sections were incubated with fluorescence-labeled secondary antibodies (Invitrogen, 1:1,000 dilution) for 1 h at RT.



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    Figure 4. Microgliosis in the OE. Staining of the OE with the macrophage marker IBA1 (green), SARS-CoV-2 nucleocapsid (red), and active phagocytic macrophages Dectin-1 <t>(Clec7A;</t> magenta). Nuclei counterstained with DAPI (blue). A with insets, Representative images of the OE from control mice. B with insets, Representative images of the OE from SARS-CoV-2–infected mice. C, Quantification of total macrophage cells (IBA1+) and phagocytic mac- rophages (IBA1+/Clec7A+) in the medial wall (septum) and turbinates. The number of combined IBA1+ cells between the septum and turbinates shows an increase that was statistically significant only in the septum (C), suggesting a higher vulnerability of this region to the infection. Statistics: multiple unpaired t test with * = p < 0.05 (Table 2). Scale bars: A and B, 1 mm; septum and turbinate insets, 100 µm.
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    Figure 4. Microgliosis in the OE. Staining of the OE with the macrophage marker IBA1 (green), SARS-CoV-2 nucleocapsid (red), and active phagocytic macrophages Dectin-1 <t>(Clec7A;</t> magenta). Nuclei counterstained with DAPI (blue). A with insets, Representative images of the OE from control mice. B with insets, Representative images of the OE from SARS-CoV-2–infected mice. C, Quantification of total macrophage cells (IBA1+) and phagocytic mac- rophages (IBA1+/Clec7A+) in the medial wall (septum) and turbinates. The number of combined IBA1+ cells between the septum and turbinates shows an increase that was statistically significant only in the septum (C), suggesting a higher vulnerability of this region to the infection. Statistics: multiple unpaired t test with * = p < 0.05 (Table 2). Scale bars: A and B, 1 mm; septum and turbinate insets, 100 µm.
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    Image Search Results


    Figure 4. Microgliosis in the OE. Staining of the OE with the macrophage marker IBA1 (green), SARS-CoV-2 nucleocapsid (red), and active phagocytic macrophages Dectin-1 (Clec7A; magenta). Nuclei counterstained with DAPI (blue). A with insets, Representative images of the OE from control mice. B with insets, Representative images of the OE from SARS-CoV-2–infected mice. C, Quantification of total macrophage cells (IBA1+) and phagocytic mac- rophages (IBA1+/Clec7A+) in the medial wall (septum) and turbinates. The number of combined IBA1+ cells between the septum and turbinates shows an increase that was statistically significant only in the septum (C), suggesting a higher vulnerability of this region to the infection. Statistics: multiple unpaired t test with * = p < 0.05 (Table 2). Scale bars: A and B, 1 mm; septum and turbinate insets, 100 µm.

    Journal: eneuro

    Article Title: Inflammatory response and defects on myelin integrity in the olfactory system of K18hACE2 mice infected with SARS-CoV-2

    doi: 10.1523/eneuro.0106-24.2024

    Figure Lengend Snippet: Figure 4. Microgliosis in the OE. Staining of the OE with the macrophage marker IBA1 (green), SARS-CoV-2 nucleocapsid (red), and active phagocytic macrophages Dectin-1 (Clec7A; magenta). Nuclei counterstained with DAPI (blue). A with insets, Representative images of the OE from control mice. B with insets, Representative images of the OE from SARS-CoV-2–infected mice. C, Quantification of total macrophage cells (IBA1+) and phagocytic mac- rophages (IBA1+/Clec7A+) in the medial wall (septum) and turbinates. The number of combined IBA1+ cells between the septum and turbinates shows an increase that was statistically significant only in the septum (C), suggesting a higher vulnerability of this region to the infection. Statistics: multiple unpaired t test with * = p < 0.05 (Table 2). Scale bars: A and B, 1 mm; septum and turbinate insets, 100 µm.

    Article Snippet: CLEC7A (Dectin-1) Rat IgG2a, κ InvivoGen (mabg-mdect) RRID: AB_2753143 1:30 Donkey anti-rat IgG Alexa Fluor 555 Thermo Fisher Scientific 1:1,000 Ctip2 Rat IgG2a Abcam (ab18465) RRID: AB_2064130 1:500 Donkey anti-rat IgG Alexa Fluor 488 and 647 Thermo Fisher Scientific 1:1,000 IBA1 Rabbit Poly.

    Techniques: Staining, Marker, Control, Infection

    Figure 5. Microgliosis in the OB. Staining of microglia with IBA1 (green), SARS-CoV-2 nucleocapsid protein (red), and active phagocytic microglia with Dectin-1 (Clec7A; magenta). Nuclei counterstained with DAPI (blue). A, Representative images of the OE from control mice. B, Representative images of the OE from SARS-CoV-2–infected mice. Microglial cell bodies show a clear hypertrophy related to an activated response. Infected microglia are observed in the ONL (dotted lines). C, Quantification of the microglia (IBA1+) and phagocytic microglia (IBA1+/Clec7A+) in the OB. Total numbers of micro- glia (IBA+) are statistically significant higher numbers in infected animals, suggesting a strong inflammation. This effect is replicated on each individual layer excepting in the MCL-IPL, which is the layer less affected by the microgliosis. Numbers of phagocytic microglia (IBA1+/Clec7A+) are significantly higher only in the GL, indicating that this layer is the most affected by inflammation. EPL, external plexiform layer; GCL, granule cell layer; GL, glomerular layer; IPL, internal plexiform layer; MCL, mitral cell layer; OB, olfactory bulb; ONL, olfactory nerve layer; RMS, rostral migratory stream (bulbar part). Statistics: multiple unpaired t test with * = p < 0.05; ** = p < 0.01 (Table 2). Scale bars: A and B, 1 mm; high magnification insets, 200 µm.

    Journal: eneuro

    Article Title: Inflammatory response and defects on myelin integrity in the olfactory system of K18hACE2 mice infected with SARS-CoV-2

    doi: 10.1523/eneuro.0106-24.2024

    Figure Lengend Snippet: Figure 5. Microgliosis in the OB. Staining of microglia with IBA1 (green), SARS-CoV-2 nucleocapsid protein (red), and active phagocytic microglia with Dectin-1 (Clec7A; magenta). Nuclei counterstained with DAPI (blue). A, Representative images of the OE from control mice. B, Representative images of the OE from SARS-CoV-2–infected mice. Microglial cell bodies show a clear hypertrophy related to an activated response. Infected microglia are observed in the ONL (dotted lines). C, Quantification of the microglia (IBA1+) and phagocytic microglia (IBA1+/Clec7A+) in the OB. Total numbers of micro- glia (IBA+) are statistically significant higher numbers in infected animals, suggesting a strong inflammation. This effect is replicated on each individual layer excepting in the MCL-IPL, which is the layer less affected by the microgliosis. Numbers of phagocytic microglia (IBA1+/Clec7A+) are significantly higher only in the GL, indicating that this layer is the most affected by inflammation. EPL, external plexiform layer; GCL, granule cell layer; GL, glomerular layer; IPL, internal plexiform layer; MCL, mitral cell layer; OB, olfactory bulb; ONL, olfactory nerve layer; RMS, rostral migratory stream (bulbar part). Statistics: multiple unpaired t test with * = p < 0.05; ** = p < 0.01 (Table 2). Scale bars: A and B, 1 mm; high magnification insets, 200 µm.

    Article Snippet: CLEC7A (Dectin-1) Rat IgG2a, κ InvivoGen (mabg-mdect) RRID: AB_2753143 1:30 Donkey anti-rat IgG Alexa Fluor 555 Thermo Fisher Scientific 1:1,000 Ctip2 Rat IgG2a Abcam (ab18465) RRID: AB_2064130 1:500 Donkey anti-rat IgG Alexa Fluor 488 and 647 Thermo Fisher Scientific 1:1,000 IBA1 Rabbit Poly.

    Techniques: Staining, Control, Infection

    Figure 6. Microgliosis in piriform cortex and TuS. Staining of microglia with IBA1 (green), SARS-CoV-2 nucleocapsid (red), and active phagocytic macro- phages with Dectin-1 (Clec7A; magenta). Nuclei counterstained with DAPI (blue). A, Representative images of the PC and TuS from control mice. B, Representative images of the PC and TuS from SARS-CoV-2–infected mice, where clear hypertrophy is observed in all cell bodies extending not only into the PC and TuS but also across the entire brain. C, Quantification of microglia (IBA1+) and phagocytic microglia (IBA1+/Clec7A+) in PC. The num- bers of microglia are statistically significantly higher in all layers of PC from infected animals proving the presence of strong inflammation. D, Quantification of microglia (IBA1+) and phagocytic microglia (IBA1+/Clec7A+) in TuS. The numbers of microglia (IBA1+) were significantly higher in Layers 2 and 3 high- lighting that inflammation affected the projection neuron layers. Numbers of phagocytic microglia (IBA1+/Clec7A+) are similar in both controls and infected mice. LOT, lateral olfactory tract; PC, piriform cortex; TuS, tubular striatum. Statistics: multiple unpaired t test with * = p < 0.05; ** = p < 0.01; *** = p < 0.001 (Table 2). Scale bars: A and B, 1 mm; high magnification insets, 200 µm.

    Journal: eneuro

    Article Title: Inflammatory response and defects on myelin integrity in the olfactory system of K18hACE2 mice infected with SARS-CoV-2

    doi: 10.1523/eneuro.0106-24.2024

    Figure Lengend Snippet: Figure 6. Microgliosis in piriform cortex and TuS. Staining of microglia with IBA1 (green), SARS-CoV-2 nucleocapsid (red), and active phagocytic macro- phages with Dectin-1 (Clec7A; magenta). Nuclei counterstained with DAPI (blue). A, Representative images of the PC and TuS from control mice. B, Representative images of the PC and TuS from SARS-CoV-2–infected mice, where clear hypertrophy is observed in all cell bodies extending not only into the PC and TuS but also across the entire brain. C, Quantification of microglia (IBA1+) and phagocytic microglia (IBA1+/Clec7A+) in PC. The num- bers of microglia are statistically significantly higher in all layers of PC from infected animals proving the presence of strong inflammation. D, Quantification of microglia (IBA1+) and phagocytic microglia (IBA1+/Clec7A+) in TuS. The numbers of microglia (IBA1+) were significantly higher in Layers 2 and 3 high- lighting that inflammation affected the projection neuron layers. Numbers of phagocytic microglia (IBA1+/Clec7A+) are similar in both controls and infected mice. LOT, lateral olfactory tract; PC, piriform cortex; TuS, tubular striatum. Statistics: multiple unpaired t test with * = p < 0.05; ** = p < 0.01; *** = p < 0.001 (Table 2). Scale bars: A and B, 1 mm; high magnification insets, 200 µm.

    Article Snippet: CLEC7A (Dectin-1) Rat IgG2a, κ InvivoGen (mabg-mdect) RRID: AB_2753143 1:30 Donkey anti-rat IgG Alexa Fluor 555 Thermo Fisher Scientific 1:1,000 Ctip2 Rat IgG2a Abcam (ab18465) RRID: AB_2064130 1:500 Donkey anti-rat IgG Alexa Fluor 488 and 647 Thermo Fisher Scientific 1:1,000 IBA1 Rabbit Poly.

    Techniques: Staining, Control, Infection